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Oxidative attack of essential cell components by reactive oxygen species has been associated with several human diseases, such as atherosclerosis, cardiovascular diseases, diabetes, liver disorders, and inflammatory rheumatic diseases. THIOBARBITURIC ACID REACTIVE SUBSTANCES (TBARS) are low-molecular-weight end products (mainly malondialdehyde, MDA) that are formed during the decomposition of lipid peroxidation products. Increased levels of TBARS have been demonstrated in these diseases. Simple, direct and accurate assays for TBARS find wide applications in research and drug discovery. BioAssay Systems' TBARS assay is based on the reaction of TBARS with thiobarbituric acid (TBA) to form a pink colored product. The color intensity at 535nm or fluorescence intensity at (ex/em = 560nm/585nm) is directly proportional to TBARS concentration in the sample. This product is for Research Use only and is not intended for diagnostic purposes.
For quantitative determination of thiobarbituric acid reactive substances (TBARS).
Method Colorimetric (535nm), or fluorometric (560/585nm) assay. A spectrophotometer or plate reader is required, but not provided.
Samples Serum, plasma, urine, saliva etc. Species All. Detects 0.1 uM
Procedure 80 min. Size 100 tests per kit