BioVision Hydrogen Peroxide Colorimetric/Fluorometric Assay Kit (K265-200) - Accurate H2O2 Detection for Cell Biology & Oxidative Stress Research

Sensitive Assay, HTS Detection method- Absorbance (570 nm)and fluorescence(Ex/Em 535/587 nm)Species reactivity- Mammalian Applications- The kit provides a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring Hâ‚‚Oâ‚‚ in biological samples. Hydrogen Peroxide is a reactive oxygen metabolic byproduct that serves as a key regulator for a number of oxidative stress-related states. Functioning through NF-κB and other factors, hydroperoxide-mediated pathways have been linked to asthma, inflammatory arthritis, atherosclerosis, diabetic vasculopathy, osteoporosis, neuro-degenerative diseases, Downâ€s syndrome and immune system diseases. BioVisionâ€s Hydrogen Peroxide Assay Kit is a highly sensitive, simple, direct and HTS-ready colorimetric and fluorometric assay for measuring Hâ‚‚Oâ‚‚ in biological samples. In the presence of Horse Radish Peroxidase (HRP), the OxiRed Probe reacts with Hâ‚‚Oâ‚‚ to produce product with color (λmax = 570 nm) and red-fluorescent (Ex/Em=535/587 nm). The kit can perform 200 reactions by fluorometric method or 100 reactions by colorimetric method. The detection limit can be as low as 2 pmol per assay (or 40 nM concentration) of Hâ‚‚Oâ‚‚ in the sensitive fluorometric assay.